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STUDIES ON THE ISOLATION OF METAPHASE CHROMOSOMES

A method for the isolation of metaphase chromosomes from mouse L1210 leukemia cells has been developed. Cells, arrested at metaphase with colchicine, were exposed to hypotonic solution and the pH was then adjusted to 5.6 to stabilize the chromosomes. The metaphase figures were subsequently disrupted...

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Autori principali: ChoraŻy, M., Bendich, A., Borenfreund, E., Hutchison, D. J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Rockefeller University Press 1963
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2106868/
https://ncbi.nlm.nih.gov/pubmed/14069802
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