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STUDIES ON ISOLATED NUCLEI : I. Isolation and Chemical Characterization of a Nuclear Fraction from Guinea Pig Liver

This article describes a method for the isolation of nuclei from guinea pig liver. It involves the homogenization of the tissue in 0.88 M sucrose-1.5 mM CaCl(2) followed by centrifugation in a discontinuous density gradient in which the upper phase is the homogenate and the lower phase is 2.2 M sucr...

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Dettagli Bibliografici
Autori principali: Maggio, Rachele, Siekevitz, Philip, Palade, George E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Rockefeller University Press 1963
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2106307/
https://ncbi.nlm.nih.gov/pubmed/14079489
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