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THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN
Nuclei can be isolated from the eggs and embryos of the sea urchin by a four step method: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest o...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The Rockefeller University Press
1962
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2106168/ https://ncbi.nlm.nih.gov/pubmed/13954643 |
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