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THE ISOLATION OF NUCLEI FROM EGGS AND EMBRYOS OF THE SEA URCHIN

Nuclei can be isolated from the eggs and embryos of the sea urchin by a four step method: (1) washing with dextrose solution until the cells become weak, (2) cytolysis in 0.002 M Mg(++) solution, (3) homogenization of the cytolyzed cells by agitation, and (4) separation of the nuclei from the rest o...

Ausführliche Beschreibung

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Bibliographische Detailangaben
1. Verfasser: Hinegardner, Ralph T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Rockefeller University Press 1962
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2106168/
https://ncbi.nlm.nih.gov/pubmed/13954643
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