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Construction, expression, and localization of a CycA::PhoA fusion protein in Rhodobacter sphaeroides and Escherichia coli.

We demonstrated the utility of Escherichia coli alkaline phosphatase, encoded by phoA, as a reporter molecule for genetic fusions in Rhodobacter sphaeroides. A portion of the R. sphaeroides cycA gene was fused to phoA, yielding a fusion protein comprising the putative signal sequence and first 10 am...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Varga, A R, Kaplan, S
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1989
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210443/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2553661/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.11.5830-5839.1989
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