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Targeted gene expression without a tissue-specific promoter: Creating mosaic embryos using laser-induced single-cell heat shock

We have developed a method to target gene expression in the Drosophila embryo to a specific cell without having a promoter that directs expression in that particular cell. Using a digitally enhanced imaging system to identify single cells within the living embryo, we apply a heat shock to each cell...

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Autori principali: Halfon, Marc S., Kose, Hiroyuki, Chiba, Akira, Keshishian, Haig
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences of the USA 1997
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC21036/
https://ncbi.nlm.nih.gov/pubmed/9177204
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