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In vitro construction of gshB::kan in Escherichia coli and use of gshB::kan in mapping the gshB locus.

The Escherichia coli structural gene for glutathione synthetase, gshB, was cloned into pBR322. Plasmids containing gshB were able to complement the glutathione requirement of a trxA gshB double mutant, and cells containing the plasmids were found to have elevated levels of glutathione synthetase. A...

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Библиографические подробности
Главные авторы: Daws, T, Lim, C J, Fuchs, J A
Формат: Artigo
Язык:Inglês
Опубликовано: 1989
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC210347/
https://ncbi.nlm.nih.gov/pubmed/2670910
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