Cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti.

A genomic library of Acetobacter aceti DNA was constructed by using a broad-host-range cosmid vector. Complementation of a spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation of a plasmid designated pAA701. Subcloning and deletion analysis of pAA701 limited the region that c...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Inoue, T, Sunagawa, M, Mori, A, Imai, C, Fukuda, M, Takagi, M, Yano, K
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1989
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210023/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2722742/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.6.3115-3122.1989
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