Purification and characterization of a novel form of 20 alpha-hydroxysteroid dehydrogenase from Clostridium scindens.

We have purified a steroid-inducible 20 alpha-hydroxysteroid dehydrogenase from Clostridium scindens to apparent homogeneity. The final enzyme preparation was purified 252-fold, with a recovery of 14%. Denaturing and nondenaturing polyacrylamide gradient gel electrophoresis showed that the native en...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Krafft, A E, Hylemon, P B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209996/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2722736/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.6.2925-2932.1989
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