Chromosomal transformation of Escherichia coli recD strains with linearized plasmids.
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transf...
Spremljeno u:
| Izdano u: | J Bacteriol |
|---|---|
| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1989
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209941/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2651408/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.5.2609-2613.1989 |
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