New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis.

To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was lysogenic for prophage phi 80 and carried a lac duplication. By sc...

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Bibliographic Details
Main Authors: Dutreix, M, Moreau, P L, Bailone, A, Galibert, F, Battista, J R, Walker, G C, Devoret, R
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC209916/
https://ncbi.nlm.nih.gov/pubmed/2651400
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