Identification of Escherichia coli DNA helicase IV with the use of a DNA helicase activity gel.
A DNA helicase activity gel was developed based on the assumption that DNA helicases could unwind double-stranded DNA in a polyacrylamide matrix. The production of single-stranded DNA was detected by staining the activity gel with acridine orange and visualizing the gel under long-wave UV light. The...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209867/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2539361/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.4.2128-2135.1989 |
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