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Cell-associated pullulanase from Bacteroides thetaiotaomicron: cloning, characterization, and insertional mutagenesis to determine role in pullulan utilization.

We have cloned a pullulanase gene from Bacteroides thetaiotaomicron. The pullulanase expressed from this clone in Escherichia coli was cell associated and soluble and had a molecular mass of 72 kilodaltons by gel filtration. Maxicell analysis of proteins coded by the cloned insert showed that a 71.6...

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Библиографические подробности
Главные авторы: Smith, K A, Salyers, A A
Формат: Artigo
Язык:Inglês
Опубликовано: 1989
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC209865/
https://ncbi.nlm.nih.gov/pubmed/2703467
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