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Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases.

The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Shyamala, V, Ames, G F
פורמט: Artigo
שפה:Inglês
יצא לאור: 1989
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC209787/
https://ncbi.nlm.nih.gov/pubmed/2646290
תגים: הוספת תג
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