Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases.
The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with...
সংরক্ষণ করুন:
| প্রকাশিত: | J Bacteriol |
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| প্রধান লেখক: | , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
American Society for Microbiology (ASM)
1989
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209787/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2646290/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.3.1602-1608.1989 |
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