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Bacillus subtilis phenylalanyl-tRNA synthetase genes: cloning and expression in Escherichia coli and B. subtilis.

The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the cor...

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Autors principals: Brakhage, A A, Putzer, H, Shazand, K, Röschenthaler, R J, Grunberg-Manago, M
Format: Artigo
Idioma:Inglês
Publicat: 1989
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC209730/
https://ncbi.nlm.nih.gov/pubmed/2492510
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