A recB recC sbcB recJ host prevents recA-independent deletions in recombinant cosmid DNA propagated in Escherichia coli.

Segments of DNA are deleted from recombinant cosmid DNAs with high frequency during propagation in standard recA Escherichia coli hosts. An attempt has been made to derive an appropriate strain of E. coli, suitable for cosmid cloning, in which such deletions do not occur. We examined the effects of...

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Dades bibliogràfiques

Publicat a:	J Bacteriol
Autors principals:	Ishiura, M, Hazumi, N, Koide, T, Uchida, T, Okada, Y
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1989
Matèries:	Research Article
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209703/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2536671/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.2.1068-1074.1989
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