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Bioluminescence Regenerative Cycle (BRC) System for Nucleic Acid Quantification Assays
A new label-free methodology for nucleic acid quantification has been developed where the number of pyrophosphate molecules (PPi) released during polymerization of the target nucleic acid is counted and correlated to DNA copy number. The technique uses the enzymatic complex of ATP-sulfurylase and fi...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2005
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2096776/ https://ncbi.nlm.nih.gov/pubmed/15882922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpc.2005.04.002 |
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