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Rational Design of Analyte Channels of the Green Fluorescent Protein for Biosensor Applications

A novel solvent-exposed analyte channel, generated by F165G substitution, on the surface of green fluorescent protein (designated His(6)GFPuv/F165G) was successfully discovered by the aid of molecular modeling software (PyMOL) in conjunction with site-directed mutagenesis. Regarding the high predict...

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Detalhes bibliográficos
Main Authors: Tansila, Natta, Tantimongcolwat, Tanawut, Isarankura-Na-Ayudhya, Chartchalerm, Nantasenamat, Chanin, Prachayasittikul, Virapong
Formato: Artigo
Idioma:Inglês
Publicado em: Ivyspring International Publisher 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2096736/
https://ncbi.nlm.nih.gov/pubmed/18071586
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