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Overinitiation of replication of the Escherichia coli chromosome from an integrated runaway-replication derivative of plasmid R1.

A 16-base-pair fragment, deletion of which completely inactivated oriC, was replaced by a temperature-dependent runaway-replication derivative (the copy number of which increases with temperature) of the IncFII plasmid R1. The constructed strains were temperature sensitive, and flow cytometry reveal...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Bernander, R, Merryweather, A, Nordström, K
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209650/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2644232/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.2.674-683.1989
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