A mollicute (mycoplasma) DNA repair enzyme: purification and characterization of uracil-DNA glycosylase.
The DNA repair enzyme uracil-DNA glycosylase from Mycoplasma lactucae (831-C4) was purified 1,657-fold by using affinity chromatography and chromatofocusing techniques. The only substrate for the enzyme was DNA that contained uracil residues, and the Km of the enzyme was 1.05 +/- 0.12 microM for dUM...
Gardado en:
| Publicado en: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1990
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209097/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2345131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.6.2979-2985.1990 |
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