Bacteriophage T4 deoxynucleotide kinase: gene cloning and enzyme purification.

Gene 1 of bacteriophage T4 has been cloned into a lambda pL expression vector, resulting in the overproduction of deoxynucleotide kinase. A procedure that includes affinity chromatography on Cibacron Blue F3GA-agarose has been used to purify milligram quantities of enzymes from a 2-liter culture. Th...

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Pubblicato in:	J Bacteriol
Autori principali:	Brush, G S, Bhatnagar, S K, Bessman, M J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209091/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2160930/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.6.2935-2939.1990
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