A method for introduction of unmarked mutations in the genome of Paracoccus denitrificans: construction of strains with multiple mutations in the genes encoding periplasmic cytochromes c550, c551i, and c553i.

A new suicide vector, pRVS1, was constructed to facilitate the site-directed introduction of unmarked mutations in the chromosome of Paracoccus denitrificans. The vector was derived from suicide vector pGRPd1, which was equipped with the lacZ gene encoding beta-galactosidase. The reporter gene was f...

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Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Van Spanning, R J, Wansell, C W, Reijnders, W N, Harms, N, Ras, J, Oltmann, L F, Stouthamer, A H
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1991
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209050/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1657872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.21.6962-6970.1991
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