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Determination of the nick site at oriT of IncI1 plasmid R64: global similarity of oriT structures of IncI1 and IncP plasmids.

The nick site at the origin of transfer, oriT, of IncI1 plasmid R64 was determined. A site-specific and strand-specific cleavage of the phosphodiester bond was introduced during relaxation of the oriT plasmid DNA. Cleavage occurred between 2'-deoxyguanosine and thymidine residues, within the 44-bp o...

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Vydáno v:J Bacteriol
Hlavní autoři: Furuya, N, Komano, T
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208999/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1917882/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.20.6612-6617.1991
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