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Cloning, sequence analysis, and functional expression of the acetyl coenzyme A synthetase gene from Methanothrix soehngenii in Escherichia coli.

In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Eggen, R I, Geerling, A C, Boshoven, A B, de Vos, W M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208970/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1680850/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.20.6383-6389.1991
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