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Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli.

We have cloned the Bacteroides fragilis TAL2480 neuraminidase (NANase) structural gene, nanH, in Escherichia coli. This was accomplished by using the cloning shuttle vector pJST61 and a partial Sau3A library of TAL2480 chromosomal inserts created in E. coli. The library was mobilized into the NANase...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Russo, T A, Thompson, J S, Godoy, V G, Malamy, M H
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1990
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208902/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2158974/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.5.2594-2600.1990
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