Evaluation of freeze-substitution and conventional embedding protocols for routine electron microscopic processing of eubacteria.

Freeze-substitution and more conventional embedding protocols were evaluated for their accurate preservation of eubacterial ultrastructure. Radioisotopes were specifically incorporated into the RNA, DNA, peptidoglycan, and lipopolysaccharide of two isogenic derivatives of Escherichia coli K-12 as re...

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Pubblicato in:	J Bacteriol
Autori principali:	Graham, L L, Beveridge, T J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208714/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2108131/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.4.2141-2149.1990
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