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Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.

A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construct...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Mukhopadhyay, P, Mukhopadhyay, M, Mills, D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1990
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208456/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2294094/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.1.477-480.1990
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