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Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas denitrificans.

The cobalamin biosynthetic pathway enzyme that catalyzes amidation of 5'-deoxy-5'-adenosyl-cobyrinic acid a,c-diamide was purified to homogeneity from extracts of a recombinant strain of Pseudomonas denitrificans by a four-column procedure. The purified protein had an isoelectric point of 5.6 and mo...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Blanche, F, Couder, M, Debussche, L, Thibaut, D, Cameron, B, Crouzet, J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208350/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1917839/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.19.6046-6051.1991
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