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Cloning, sequencing, and expression of the gene coding for bile acid 7 alpha-hydroxysteroid dehydrogenase from Eubacterium sp. strain VPI 12708.

Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-k...

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Détails bibliographiques
Publié dans:J Bacteriol
Auteurs principaux: Baron, S F, Franklund, C V, Hylemon, P B
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1991
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208130/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1856160/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.15.4558-4569.1991
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