A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.

By using a gene library of Bacillus caldolyticus constructed in phage lambda EMBL12 and selecting for proteolytically active phages on plates supplemented with 0.8% skim milk, chromosomal B. caldolyticus DNA fragments that specified proteolytic activity were obtained. Subcloning of one of these frag...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	van den Burg, B, Enequist, H G, van der Haar, M E, Eijsink, V G, Stulp, B K, Venema, G
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1991
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208060/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1905714/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.13.4107-4115.1991
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