A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.

By using a gene library of Bacillus caldolyticus constructed in phage lambda EMBL12 and selecting for proteolytically active phages on plates supplemented with 0.8% skim milk, chromosomal B. caldolyticus DNA fragments that specified proteolytic activity were obtained. Subcloning of one of these frag...

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Bibliographic Details
Main Authors: van den Burg, B, Enequist, H G, van der Haar, M E, Eijsink, V G, Stulp, B K, Venema, G
Format: Artigo
Language:Inglês
Published: 1991
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC208060/
https://ncbi.nlm.nih.gov/pubmed/1905714
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