Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae.

An auxotrophic mutant of Methanococcus voltae was isolated that required coenzyme M (CoM) for growth. With the mutant as a recipient, conditions were developed that allowed the introduction of wild-type CoM+ DNA into the mutant methanogen via electroporation. This method also allowed the rescue of b...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Micheletti, P A, Sment, K A, Konisky, J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1991
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207953/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1904435/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.11.3414-3418.1991
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