Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae.
An auxotrophic mutant of Methanococcus voltae was isolated that required coenzyme M (CoM) for growth. With the mutant as a recipient, conditions were developed that allowed the introduction of wild-type CoM+ DNA into the mutant methanogen via electroporation. This method also allowed the rescue of b...
Sparad:
| I publikationen: | J Bacteriol |
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| Huvudupphov: | , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
American Society for Microbiology (ASM)
1991
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207953/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1904435/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.11.3414-3418.1991 |
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