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Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli.

A gene library for Clostridium acetobutylicum NCIB 2951 was constructed in the broad-host-range cosmid pLAFR1, and cosmids containing the beta-galactosidase gene were isolated by direct selection for enzyme activity on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) plates after conjugal trans...

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Published in:J Bacteriol
Main Authors: Hancock, K R, Rockman, E, Young, C A, Pearce, L, Maddox, I S, Scott, D B
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1991
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207901/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850729/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.10.3084-3095.1991
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