Purification and properties of tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli.

The aroH gene of Escherichia coli, which encodes the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase isoenzyme of the common aromatic biosynthetic pathway, was cloned behind the tac promoter in expression plasmid pKK223-3. The enzyme was overexpressed, purified to homogenei...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Ray, J M, Bauerle, R
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1991
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207719/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1672127/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.6.1894-1901.1991
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