Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus.

We describe the self-selection of replication origins of undescribed cryptic plasmids from Thermus aquaticus Y-VII-51B (ATCC 25105) and a Thermus sp. strain (ATCC 27737) by random insertion of a thermostable kanamycin adenyltransferase cartridge. Once selected, these autonomous replication origins w...

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Xehetasun bibliografikoak

Argitaratua izan da:	J Bacteriol
Egile Nagusiak:	Lasa, I, de Grado, M, de Pedro, M A, Berenguer, J
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1992
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207595/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1400194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.20.6424-6431.1992
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