A Surface-Focused Biotinylation Procedure Identifies the Yersinia pestis Catalase KatY as a Membrane-Associated but Non-Surface-Located Protein

This study identified major surface proteins of the plague bacterium Yersinia pestis. We applied a novel surface biotinylation method, followed by NeutrAvidin (NA) bead capture, on-bead digestion, and identification by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The use of stachyose d...

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Main Authors: Myers-Morales, Tanya, Cowan, Clarissa, Gray, Michael E., Wulff, Christine R., Parker, Carol E., Borchers, Christoph H., Straley, Susan C.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2007
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Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2074897/
https://ncbi.nlm.nih.gov/pubmed/17644638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02968-06
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