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Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis.

The dnaB gene of Escherichia coli encodes an essential DNA replication enzyme. Fueled by the energy derived from the hydrolysis of ATP to ADP+P(i), this enzyme unwinds double-stranded DNA in advance of the DNA polymerase. While doing so, it intermittently stimulates primase to synthesize an RNA prim...

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Published in:J Bacteriol
Main Authors: Shrimankar, P, Stordal, L, Maurer, R
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207482/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1332941/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.23.7689-7696.1992
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