Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors.

On the basis of an RSF1010-derived broad-host-range vector, three different systems which enable positive detection and isolation of insertion sequence (IS) elements from gram-negative bacteria were constructed. Vectors pSUP104-pheS, pSUP104-rpsL, and pSUP104-sac were used successfully in a number o...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Simon, R, Hötte, B, Klauke, B, Kosier, B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207288/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1847366/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.4.1502-1508.1991
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