Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein.

Mini-F plasmids cannot replicate in Escherichia coli strains (delta rpoH) lacking sigma 32, presumably because transcription of the repE gene encoding the replication initiator protein (RepE protein) depends mostly on RNA polymerase containing sigma 32. We have isolated and characterized mini-F muta...

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Bibliographic Details
Main Authors: Kawasaki, Y, Wada, C, Yura, T
Format: Artigo
Language:Inglês
Published: 1991
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC207225/
https://ncbi.nlm.nih.gov/pubmed/1991708
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