High-level expression of Escherichia coli NADPH-sulfite reductase: requirement for a cloned cysG plasmid to overcome limiting siroheme cofactor.

The flavoprotein and hemoprotein components of Escherichia coli B NADPH-sulfite reductase are encoded by cysJ and cysI, respectively. Plasmids containing these two genes overexpressed flavoprotein catalytic activity and apohemoprotein by 13- to 35-fold, but NADPH-sulfite reductase holoenzyme activit...

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Hlavní autoři: Wu, J Y, Siegel, L M, Kredich, N M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC207191/
https://ncbi.nlm.nih.gov/pubmed/1987123
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