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A comparison of an improved o-phthalaldehyde fluorometric method and high pressure liquid chromatography in the determination of brain 5-hydroxyindoles of rats treated with L-tryptophan and p-chlorophenyl-alanine.

1 The determination of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) by reaction with o-phthalaldehyde (OPT) in the presence of cysteine and subsequent fluorometry was compared with determination by a new high pressure liquid chromatography (h.p.l.c.) electrochemical method. 2 T...

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Autores principales: Curzon, G., Kantamaneni, B. D., Tricklebank, M. D.
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 1981
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2071667/
https://ncbi.nlm.nih.gov/pubmed/6165422
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