Cloning, sequence analysis, and overexpression of Escherichia coli folK, the gene coding for 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase.

The gene coding for the Escherichia coli enzyme 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase has been cloned and sequenced. This gene, designated folK, codes for a protein of 159 amino acids, including an amino-terminal methionine. The protein was overexpressed in E. coli MC4100 by cloning th...

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Publié dans:	J Bacteriol
Auteurs principaux:	Talarico, T L, Ray, P H, Dev, I K, Merrill, B M, Dallas, W S
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1992
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207135/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1325970/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.18.5971-5977.1992
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