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Isolation and characterization of a DNA replication origin from the 1,700-kilobase-pair symbiotic megaplasmid pSym-b of Rhizobium meliloti.

A 4-kb fragment active as an autonomously replicating sequence (ARS) from the Rhizobium meliloti symbiotic megaplasmid pSym-b was isolated by selecting for sequences that allowed a normally nonreplicative pBR322 derivative to replicate in R. meliloti. The resulting Escherichia coli-R. meliloti shutt...

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Detalles Bibliográficos
Main Authors: Margolin, W, Long, S R
Formato: Artigo
Idioma:Inglês
Publicado: 1993
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC206766/
https://ncbi.nlm.nih.gov/pubmed/8407832
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