Identification of tlc and gltA mRNAs and determination of in situ RNA half-life in Rickettsia prowazekii.
RNAs of Rickettsia prowazekii, an obligate intracytoplasmic bacterium, have been identified and analyzed by an RNase protection assay. Total RNA, a mixture of host cell RNA and rickettsial RNA, was isolated from rickettsia-infected mouse L929 cells by the hot-phenol method. After hybridization with...
Gespeichert in:
| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1993
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206636/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7690026/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.17.5725-5727.1993 |
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