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Purification of CREB to Apparent Homogeneity; Removal of Truncation Products and Contaminating Nucleic Acid
The cAMP response element binding protein (CREB) is a mammalian transcription factor which regulates the expression of many cellular genes. CREB is commonly expressed in E. coli and purified by heat-extraction followed by affinity chromatography. We have discovered that although this purification yi...
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| Main Authors: | , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2007
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2066201/ https://ncbi.nlm.nih.gov/pubmed/17703949 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.06.011 |
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