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Reconstitution of an efficient thymidine salvage pathway in Saccharomyces cerevisiae

The budding yeast Saccharomyces cerevisiae is unable to incorporate exogenous nucleosides into DNA. We have made a number of improvements to existing strategies to reconstitute an efficient thymidine salvage pathway in yeast. We have constructed strains that express both a nucleoside kinase as well...

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Autors principals: Vernis, Laurence, Piskur, Jure, Diffley, John F. X.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC206486/
https://ncbi.nlm.nih.gov/pubmed/14500848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng121
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