Purification and characterization of DNA polymerases from Bacillus species.

DNA polymerases from Bacillus stearothermophilus, Bacillus caldotenax, and Bacillus caldovelox were purified by chromatography on DEAE-cellulose, phosphocellulose, and heparin-Sepharose and obtained in high yield. The enzyme preparations are free of exo- and endonuclease activities. Additional purif...

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Bibliographic Details
Main Authors: Sellmann, E, Schröder, K L, Knoblich, I M, Westermann, P
Format: Artigo
Language:Inglês
Published: 1992
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC206219/
https://ncbi.nlm.nih.gov/pubmed/1320608
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