Targeted insertion of selenocysteine into the alpha subunit of formate dehydrogenase from Methanobacterium formicicum.
Selenocysteine incorporation into proteins is directed by an opal (UGA) codon and requires the existence of a stem-loop structure in the mRNA flanking the UGA at its 3' side. To analyze the sequence and secondary-structure requirements for UGA decoding, we have introduced mutations into the fdhA gen...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1992
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206140/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1531049/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.3.659-663.1992 |
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