Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity.

We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia c...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Barth, P T, Bolton, L, Thomson, J C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205901/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1556080/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.8.2612-2619.1992
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