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Staphylococcal phosphoenolpyruvate-dependent phosphotransferase system: molecular cloning and nucleotide sequence of the Staphylococcus carnosus ptsI gene and expression and complementation studies of the gene product.

A digoxigenin-labeled DNA probe that was complementary to the gene ptsH and the beginning of the gene ptsI was used to clone a 3.2-kb HincII-BamHI restriction fragment containing the complete ptsI gene of Staphylococcus carnosus. The restriction fragment was cloned in the antisense orientation to th...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Kohlbrecher, D, Eisermann, R, Hengstenberg, W
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205840/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1551842/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.174.7.2208-2214.1992
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