Purification of a nocardicin A-sensitive LD-carboxypeptidase from Escherichia coli by affinity chromatography.

An LD-carboxypeptidase releasing the terminal D-Ala from UDP-MurNAc-L-Ala-D-Glu-m-A2pm-D-Ala (UDP-MurNAc-tetrapeptide) was purified from Escherichia coli to biochemical homogeneity and characterized biochemically. Final purification was achieved by nocardicin A-Sepharose affinity chromatography. An...

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Bibliographic Details
Main Authors: Ursinus, A, Steinhaus, H, Höltje, J V
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC205735/
https://ncbi.nlm.nih.gov/pubmed/1729236
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